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Description
Human SSTR2 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be ultrasonically disrupted or repeatedly frozen and thawed. Finally, the homogenate is centrifuged at 5000×g for 5-10 minutes and the supernatant is collected for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a somatostatin receptor type 2 (SSTR2) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of somatostatin receptor type 2 (SSTR2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Somatostatin receptor type 2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Somatostatin receptor 2 is a protein encoded by the SSTR2 gene. The SSTR2 gene is also present in most other vertebrates. Somatostatin receptor 2 (SSTR2) belongs to the G protein-coupled receptor family and is most abundantly expressed in the pancreas (including α and β cells), but is also expressed in other tissues such as the brain and kidney, and to a lesser extent in the jejunum, colon, and liver. In the pancreas, it binds to somatostatin, inhibiting the secretion of pancreatic enzymes. During development, it stimulates neuronal migration and axonal growth. Somatostatin receptor 2 is expressed in most tumors. Patients with neuroendocrine tumors that overexpress somatostatin receptor 2 have a better prognosis. SSTR2 overexpression can be exploited to selectively deliver radioactive peptides to tumors for detection or destruction. SSTR2 also has the ability to stimulate apoptosis in many cells, including cancer cells. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates and other biological fluids |
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4.7 ★★★★★
Based on 16 reviews
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Product Reviews
★★★★★ 5
The best laptop sleeve I've ever owned!
Color: Black, Size: 13-inch for New MacBook Air & Pro
I love this sleeve. Every time I get a new laptop, I buy this exact one. I have one for my work laptop and one for my personal laptop.
It’s made from a high-quality outer shell with an equally nice inner lining that’s velvety soft and keeps expensive devices safe. The sleeve is padded on all sides, so no matter what angle you drop it from (and I have), your laptop stays secure. If you have a favorite bag that doesn’t include a padded laptop compartment, this will quickly become your best friend.
It also features a generous front zippered pocket for chargers, hard drives, and cables. Check out my video to see the capacity—you can easily fit a full-size charger, multiple cables, and external hard drives.
A couple of other things I love about this case: it’s easy to clean (just a damp cloth with a little soap), and it’s incredibly durable. I bought my first one almost 10 years ago, and it’s still going strong. The zipper action is still flawless.
For reference, the sleeve in my video is holding a 2025 13-inch MacBook Air, but I have the same sleeve for a 13-inch MacBook Pro, and it fits both beautifully.
I can’t recommend this sleeve enough.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 14, 2026
★★★★★ 5
Sturdy, high quality, and protective
Color: Green, Size: 15-inch for New MacBook Air
This is my third TomToc laptop sleeve. Each time, I bought another, less expensive brand first and returned it. TomToc offers the best protection in a soft-shell sleeve.
Immediately when you open it, you feel the higher quality material and density. It’s not heavy, but it is thick padded and doesn’t easily bend with pressure. The zipper is built to last, sturdy, and easy to operate without that chintzy feeling from cheap zippers.
If you pay attention to the construction, you quickly see why it’s about double in price. There is an inner pad to protect the laptop from not just drops and shock, but also from the zipper. The corners are reinforced and add extra protection. It is definitely worth double the price.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 11, 2026
★★★★★ 5
Great Protection
Color: Orange, Size: 13-inch for New MacBook Air & Pro
This is a very nice, protective case. The material is thick and cushions the laptop great! Computer fits nice and cozy; great fit. I love the bright orange! I highly recommend.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 23, 2026
★★★★★ 5
A sold, attractive, case.
Color: Navy Blue, Size: 13-inch for New MacBook Air & Pro
This case looks great, has a nice, lively color scheme, and seems to be reasonably protective and sturdy. I wouldn’t toss a laptop around with only this as protection, but I feel fairly confident in it for small drops. The fabric is easy to clean and has held up well. You can charge without taking it out of the case thanks to a zipper, and it doesn’t add much bulk, making it quite portable. All in all a good balance of protection, cost, and style.
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Reviewed in the United States on June 2, 2026
★★★★★ 5
I would rescue it from a fire even if it were empty. I'm kidding but I do like this sleeve a lot.
Color: Orange, Size: 13-inch for New MacBook Air & Pro
Fits perfectly. Superb padding. The corner padding inspires confidence-not that I want to test it! The orange color is-chef's kiss-beautiful. A perfect contrast to the midnight color of my MacBook. I could gush about that stuff all day. But you all want to know about the other stuff.
Zippers: Two YKK no snag bi-directional closers for the main opening and a solo no snag YKK for the side pocket.
Outside: Feels like tactical nylon type fabric. I haven't and won't intentionally test its stain resistance. But I suspect it would clean up just fine.
Interior: A very soft microfiber like interior covering an odor free padding that is firm yet forgiving. There is a recess you push your laptop into making for a snug fit that, like I wrote earlier, inspires confidence. This sleeve is larger than some competing brands but seeing the recess in person explains why.
Having touched on the size of this sleeve, it maintains the svelteness of my MacBook Air. Yes, it is thicker than some other sleeves, by a smidge. However the additional protection suggested but that smidge, that minute extra ish is worth it to me. The sleeve fits in my travel backpack, so that's all good.
Lastly, I would consider putting a tablet or phone in the sidepocket but not anything that would concentrate the any energy that dissipates during a fall. Had I designed this product I wouldn't have included the side pocket. It's great for documents and thin things but the sell page suggests chargers. That seems like inviting risk. You do you.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 22, 2026
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